Fig 1: Cuprizone-induced MsrA-/- mice exhibit aggravated demyelination and impaired motor coordination. (A) detection of MsrA protein expression in WT mice and MsrA-knockout mice by Western blot analysis; (B) Oil Red O staining of demyelination in corpus callosum of mice (× 200, scale bar = 50 µm); (C) analysis of the motor coordination ability of mice by the rotarod test; (D) detection of Olig2-positive cells in corpus callosum by immunofluorescence assay (× 200, scale bar = 50 µm); (E) detection of CNP-positive cells in corpus callosum by immunofluorescence assay (× 400, scale bar = 25 µm). *p < 0.05, compared to WT mice; #p < 0.05, compared to WT mice treated with cuprizone. The values are measurement data, expressed as mean ± standard deviation, and the comparisons between multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test, N = 10.Abbreviations: MsrA, Methionine sulfoxide reductase; WT, wild type; CNP, C-type natriuretic peptide; ANOVA, analysis of variance.